Analysis Of Anti C Alloantibodies

Sensitive assays such as Luminex with single alleles permit a detailed analysis of antibody specificity patterns to assess HLA mismatch acceptability of potential donors. An important component is the determination of the epitope repertoire on the HLA molecular surface because this information may lead to a more efficient epitope-based matching algorithm aimed to control antibody-mediated rejection. HLAMatchmaker is a structurally based matching program that considers each HLA antigen as a string of epitopes represented by short linear sequences involving polymorphic amino acid residues originally referred to as triplets in antibody-accessible positions [1].

E-mail address: duquesnoyr upmc. Under the auspices of the 15 th International Histocompatibility Workshop, we are conducting a multilaboratory collaborative project to Analysis Of Anti C Alloantibodies these epitopes and also to determine how often they induce specific Water Essay in patients with rejected kidney transplants.

Introduction

This report addresses the reactivity of post-allograft nephrectomy sera tested for DRB antibodies with Luminex assays using single alleles. This analysis was performed for 19 informative kidney transplant cases contributed by 13 laboratories worldwide. Although these antigens might share an epitope recognized by these antibodies, this interpretation is incorrect.

Conversely, we analyzed eight patients sensitized by a single DR1 mismatch that has no associated DR All of these patients reacted also with DR51, and this could be explained only by antibodies against the shared 96EV eplet.

Utwórz poprawne odniesienie w stylach APA, MLA, Chicago, Harvard i wielu innych

These findings demonstrate that 96EV represents a highly immunogenic epitope that can induce cross-sensitization between antigens encoded by the different DRB loci, and also that DR51 is important in determining DRB mismatch acceptability of potential donors. This analysis has also demonstrated that antibody responses are restricted to a few epitopes on these immunizing DR antigens. Published by Elsevier Inc. All rights reserved. The eplet version of HLAMatchmaker permits a more complete assessment of the epitope repertoire.

Related subjects

Many sensitized patients have antibodies induced by a transplant, and a detailed analysis of antibody specificity patterns provides a better understanding of the humoral immune response to mismatched HLA antigens of the transplant donor. Serum antibodies are more readily detectable after the transplant has been removed because allograft tissue can absorb circulating donor-specific HLA antibodies. Sera from patients from whom the rejected kidney transplant had been removed have antibodies specific for a restricted number of HLAMatchmaker defined epitopes on immunizing donor HLA antigens [3].

Under auspices of the 14 th and 15 th International Histocompatibility Workshops we initiated a multilaboratory Analysis Of Anti C Alloantibodies project to characterize these epitopes and also how often they induce specific antibodies in patients with rejected kidney transplants. The latter would provide an assessment of the epitope immunogenicity.

The 14 th Workshop project has generated preliminary information about class I epitope immunogenicity [4]. The Luminex assay with single HLA alleles offers new opportunities to analyze HLA antibody reactivity patterns in much more precise detail.

More than 25 laboratories worldwide are participating in the 15 th Workshop project on epitope immunogenicity. Approximately informative allograft nephrectomy cases have been submitted so far, and many of them have yielded interesting information that have led to a better understanding of antibody recognition of HLA epitopes.

Almost all sera from DR2-sensitized patients reacted also with DR1. Although an obvious interpretation could be that DR1 and DR2 might have a shared epitope, the HLAMatchmaker analysis clearly shows a different pattern of epitope sharing. Subjects and methods.]

Analysis Of Anti C Alloantibodies - remarkable

Pisani, G. Lawless, Jose Mendez, Marc A. Silver, Ruta Radvany, and John A. Methods: Sixteen Group 1 of patients awaiting cardiac transplantation were found to be sensitized. These patients underwent plasmapheresis followed by 20 gm of intravenous immunoglobulin G IVIG immediately prior to cardiac transplantation. Analysis Of Anti C Alloantibodies

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